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KMID : 1145120180160040579
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Asian Journal of Beauty and Cosmetology
2018 Volume.16 No. 4 p.579 ~ p.589
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Effects of Acetyl Glutamine on the Improvement of Skin Barrier and Inhibition of Senescence
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Jang Hyun-Hee
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Abstract
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Purpose: The purpose of this work is to investigate the effects of acetyl glutamine (NAG) as a cosmetic ingredient-the effect of the inhibition of senescence and enhancing skin barrier-through cell-based assays.
Method: We confirmed the effects of NAG using cell viability, SA-β-galactosidase assay, western blotting and quantitative real-time PCR.
Results: NAG did not affect cell viability. NAG at 20 mM showed a significant protective effect against H2O2-induced loss of cell viability at 100, 200 μM. It was found that 20 mM NAG reduced senescence cells in H2O2-induced HaCaT far more than glutamine (Gln) in SA-β-gal positive cells. NAG at 20 mM increased expressions of keratin 1 and involucrin remarkably more than Gln. It indicates that NAG has better efficacy in facilitating differentiation of epidermal keratinocyte than Gln. Also, NAG reduced nuclear accumulation of p53, a homotetrameric transcription factor, and expression of p21 in HDFs with H2O2. NAG decreases expression levels of matrix metallopeptidase 1 (MMP1 ) mRNA in HDFs with H2O2. The H2O2-induced decrease in the levels of collagen, type I, alpha 1 (COL1A1 ) expression were significantly increased by treatment with NAG. The result indicates that NAG effectively controls senescence cells triggered by H2O2. As a result, it was found that NAG was effective in maintaining moisture of stratum corneum, the most important function of skin barrier.
Conclusion: As shown earlier, this work tested NAG's effects of the inhibition of senescence and enhancing skin barrier. These findings suggest that NAG might be used as a cosmetic ingredient.
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KEYWORD
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Acetyl glutamine, Skin barrier, Senescence, HaCaT, HDFs
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